Conversion of Fishery Waste to Proteases by Streptomyces speibonae and Their Application in Antioxidant Preparation

Proteinaceous wastes from the fishery process are an abundant renewable resource for recovery of a variety high-value products. This work attempted to utilize several proteinaceous produce proteases using Streptomyces speibonae TKU048 strain. Among different possible carbon and nitrogen sources, protease productive activity S. was optimal on 1% tuna head powder. Further, casein/gelatin/tuna powder zymography crude enzyme revealed presence three/nine/six proteases, respectively. The crude-enzyme cocktail exhibited best proteolytic at 70 °C pH = 5.8. Sodium dodecyl sulfate strongly enhanced cocktail, whereas FeCl3, CuSO4, ethylenediaminetetraacetic acid could completely inhibit activity. Additionally, efficiently enhance 2,2-diphenyl-1-picrylhydrazyl 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities all tested materials including head, viscera, meat fish; tilapia meat, shell shrimp; squid pen; crab shell; soybean. Taken together, potential in reclamation production antioxidant preparation.